773311 Introduction into crystallography and NMR spectroscopy of proteins (in Eng.)


Art
Vorlesung
Semesterstunden
2
Vortragende/r (Mitwirkende/r)
Kosma, Paul
Organisation
Abteilung für Organische Chemie (DCH/OC)
Angeboten im Semester
Sommersemester 2018
Unterrichts-/ Lehrsprachen
Englisch

Lehrinhalt

Structural features of amino acids, peptides and proteins
Theory of x-ray diffraction from crystals
Theory of crystallization, practical aspects
Data collection, phasing, molecular replacement and structure refinement/validation
Databases and case studies
Basic principles of NMR spectroscopy
Multidimensional NMR
NMR Structure determination (assignment, Noe)
Isotope labeling of proteins, assignment strategies, residual dipolar couplings
Model building, structure refinement and validation
Protein Dynamics (Relaxation phenomena)
Protein-ligand structures (TrNOe, STD)
Comparison of X-ray and NMR structures
Applications in metabolomics and high-throughput techniques
.

Inhaltliche Voraussetzungen (erwartete Kenntnisse)

Basic knowledge of biochemistry and spectroscopy. The lecture is recommended for advanced master study students and PhDs
.

Lehrziel

The students will have obtained a basic knowledge in structural biology. In particular, they should be able to assess the quality of published protein structural data and models and get hands-on experience in using 3-D models from the protein database
.
Noch mehr Informationen zur Lehrveranstaltung, wie Termine oder Informationen zu Prüfungen, usw. finden Sie auf der Lehrveranstaltungsseite in BOKUonline.