791357 Up- und downstream-processing (in Eng.)


Art
Vorlesung
Semesterstunden
3
Vortragende/r (Mitwirkende/r)
Jungbauer, Alois , Kasper, Cornelia
Organisation
Institut für Biotechnologie
Angeboten im Semester
Wintersemester 2017/18
Unterrichts-/ Lehrsprachen
Englisch

Lehrinhalt

Upstream processing
Overview and introduction to
- production hosts
- medium/supplements
- cultivation modes and feeding strategies
- consumables/equipment/disposables
- examples of different production systems/bioreactors/process modes for biotechnological and biopharmaceutical products

Downstream Processing
•Host cell derived impurities (Protein, DNA, endotoxins, critical impurities)
•Flocculation, Precipitation, Crystallization
•Extraction
•Protein refolding
•Removal of impurities and adventitious agents
•Virus/TSE inactivation strategies
•Continuous purification strategies (SMB, CCE, CCC)
•Purification of antibodies, plasmids, viruses, and virus like particles
•Protein stabilisation

Inhaltliche Voraussetzungen (erwartete Kenntnisse)

Basic chemical engineering knowledge is requested. This includes basics in heat, mass, momentum transfer, and basics in thermodynamics. Solutions of differential equations (ODE solver). Furthermore basic knowledge in bioporocess engineering and cell culture technology as well as cell biology is desirable

Lehrziel

After successful completion of this course, students are able:
-to select suitable medium composition and supplements, choice of production host, select suitable bioreactor, cultivation mode and feeding strategy as well as unit operations for a given biotechnological production problem, such as manufacturing of bioactive compounds (eg protein, antibody vaccine)
-to select appropriate quality and in-process control methods and standards for biotechnological manufacturing.
Noch mehr Informationen zur Lehrveranstaltung, wie Termine oder Informationen zu Prüfungen, usw. finden Sie auf der Lehrveranstaltungsseite in BOKUonline.