3rd International Conference on

Analysis of Microbial Cells

at the Single Cell Level

May, 26th - 29th, 2005

Hotel Panhans, Semmering, Austria



 
 

Aims and Scope

Registration

Scientific Committee

Programme NEW

Deadlines
extended poster submission deadline

Organisation

Venue and Travel

Grants

Sponsors




Aims and Scope

Cultures of microbial cells were traditionally viewed as uniform populations, which could be adequately described by analysing either the culture supernatant or a lysate of all the cells in the population. However, with the advent of flow cytometry and image analysis it became increasingly clear that even pure populations derived from a single cell are far from homogeneous. In recent years new methods for single cell analysis have improved our possibilities for in depth analysis of what is happening in cells and why. Genetic probes and markers such as luminescent fusion proteins, the detection of molecular interactions by Fluorescence Energy Transfer and methods that analyse the activation status of cells or even specific proteins are just a few examples. Clearly cytomics, if defined as the global analysis of cell function at the cellular level, becomes an important addition to the available tools in cell biology. Not only does it link all these methods by looking at the outcome of their interaction as a cellular response, it also emphasises the interdisciplinary nature of cell science.

Due to the commercialisation of small, easy-to-use and cheap instruments these methods have now found their way into the routine lab for the analysis of cellular function, cell-cell interactions, the dynamics, as well as the heterogeneity of populations. The present meeting will follow up the two previous ones by presenting new developments and their application to the analysis of bacterial, yeast, fungal and animal cells in basic research, biotechnology and the environment.

Preliminary Programme (minor modifications still possible)

for a pdf-file of the program click here

Thursday, May 26th, 2005

14:30-14:45    Opening of the Conference

Session I: New Instruments and Techniques

14:45-15:15    Byron Brehm-Stecher, Iowa State University, USA
                        Single Cell Microbiology: An Overview of New Technologies

15:15-15:45    Gerhard Schütz, Johannes Kepler University, Linz, Austria
                        Ultra-sensitive Microscopy on living T cells

15:45-16:15     Frederick Balagadde, California Institute of Technology, USA
                        Long-term Monitoring of Bacteria Undergoing Programmed Oscillations in a Microchemostat

16:50-17:10    Mikal Heldal, University Bergen
                        Single cell analysis: X-ray microanalysis in TEM

17:10-17:30    Nelly Henry, Institut Curie, France
                        Flow cytometry to explore abiotic surfaces colonization

17:30-17:50    Tobias Preckel, Agilent
                        Quality control of siRNA, optimizing it's transfection efficiency and monitoring CD4 gene silencing effect with a                                         microfluidic chip device

17:50-18:10    Howard Shapiro, USA
                    “Cellular Astronomy” – An Inexpensive Cytometric Approach to Microbial Detection and Characterization

20:00-21:30     Dessert and Drinks in the Poster Room

Friday, May 27th, 2005

Session II: Single Cell Approaches to Genomics and Proteomics

8:30-9:00    TBA
                    Single Cell Microgenomics

9:00-9:30    Norman Dovichi, University of Washington, Seattle, USA
                    Single Cell Proteomics

9:30-10:00    Masato Miyake, AIST, Japan
                    Transfection Microarrays for analysis of intracellular molecular networks

10:00-10:20    Michael Pogson, University of Exeter, UK
                    Detection of differential gene expression in individual Salmonella biofilm cells using single copy GFP+ reporter
                    constructs

10:20-10:40    Roy Bongaerts, Institute of Food Research, Norwich, UK
                    Can the concentration of a transcriptional activator cause Non Genetic Variation in Salmonella typhimurium?


Session III: Cell Biology

11:10-11:50    Keynote Lecture: William Bentley, University of Maryland, USA
                    Wiring and Rewiring the Circuits of Quorum Sensing


11:50-12:20    Richard Daniel, University of Oxford, UK
                    Bacterial cell wall composition and morphogenesis

12:20-12:50    Danilo Porro, University of Milano-Bicocca, Italy
                    Connection between growth, cell cycle progression and metabolism in budding yeast:  the role of Sfp1

14:30-14:50    Killian Stöcker, University of Vienna, Austria
                    Single Cell Tools Identified the Yet Uncultured Crenothrix polyspora Cohn as a Novel Filamentous Methanotroph With
                    an Unusual Methane Monooxygenase

14:50-15:10    Regina Voglauer, Universität für Bodenkultur, Austria
                    Analysis of the effect of Overexpression of SNEV on normal human endothelial cells in vitro by flow cytometric methods

15:10-15:30    Katja Prutej, Applied Biocatalysis Competence Centre, Graz, Austria
                    Development of a microscope-based screening platform for the measurement of enzyme activities in single bacterial cells

15:30-15:50    Sarah Baatout, Belgian Nuclear Research Centre, Belgium
                    Physiological variations in bacteria under stress conditions: a flow cytometry approach


Session IV: Biotechnological Applications


16:40-17:10    Andreas Schmid, University Dortmund, Germany
                    The efficiency of the microbial cell as oxidation catalyst in organic chemistry

17:10-17:30    Friedrich Srienc, University of Minnesota, USA
                    Investigation of the growth physiology of cell populations in a cytostat

17:30-17:50    Thomas Walther, Technical University Dresden, Germany
                    What is the Construction Principle of Yeast Mycelia?

17:50-18:10   
Mohammed Al-Rubeai, University of Birmingham, UK
                    Single cell analysis of growth and death in cultured animal cells



20:00-21:30     Dessert and Drinks in the Poster Room


Saturday, May 28th, 2005

8:30-8:50    
Alvin Nienow, University of Birmingham, UK
                    The use of Flow Cytometry to study mixing issues in the Scale-Up of high cell density fed-batch E.coli  fermentations

8:50-9:10    Thomas Maskow, Technical University Dresden, Germany
                    Reaction heat and bacterial DNA content as control parameters for continuous polyhydroxyalkanoate synthesis

9:10-9:30   
Minoska Valli, Universität für Bodenkultur, Austria
                    Intracellular pH distribution in Saccharomyces cerevisiae cell populations analyzed by flow cytometry


Session V: Environmental Applications

9:30-10:00    Michael Wagner, University Vienna, Austria
                    New tools for identification and ecophysiological characterization of uncultured prokaryotes on a single-cell level

10:00-10:30    Masao Nasu, Osaka University, Japan
                    New approach for the detection of microbes in aquatic environments

11:00-11:20    Susann Müller, UFZ Centre for Environmental Research, Leipzig, Germany
                    Population profiles of a stable, commensalistic bacterial culture grown with toluene under sulphate-reducing conditions

11:20-11:40    Michael Berney, Swiss Federal Institute for Environmental Science and Technology, Switzerland
                    Survival and Injury of enteric bacteria exposed to Solar Disinfection (SODIS), monitored with flow cytometry

11:40-12:00    Michel Denis, Université de la Mediterranée, Marseille, France
                    Short-term variability of heterotrophic bacteria of the northwestern Mediterranean in late summer 2004. Evidence for                             pulsed mineralisation in the water column

12:00-12:20    Rodolfo Paranhos, University of Brazil, Brazil
                    From rivers to the deep sea: patterns in bacterioplankton sub-groups as revealed by flow cytometry


Session VI: Screening and Sorting

14:30-15:00    Hazel Davey, University of Wales, Aberystwyth, UK
                    Applications of flow cytometry and cell sorting in determination of microbial viability

15:00-15:30    John Löfblom, KTH, Sweden
                    Staphylococcus surface display in combinatorial bioengineering

15:30-15:50    Colin Ingham, Wageningen University, The Netherlands
                    Using a porous ceramic to build a “Better Petri Dish" for High-throughput characterisation of micro-organisms

15:50-16:10    Dethardt Müller, Universität für Bodenkultur, Austria
                    Serum-free Sorting of High Producing Recombinant CHO Cells

16:40-17:00     Closure of Meeting and Final Discussion

17:00        Keynote Lecture: Xavier Darzacq, Albert Einstein College of Medicine, Yeshiva University, USA
                    A dynamic analysis of mammalian gene expression and mRNA nuclear mobility

20:00        Gala Dinner

Abstract Submission

To submit an abstract please send an MS-Word file as attachment to singlecell@boku.ac.at. Please specify the two sessions that you think relate best to your work. All submitted abstracts will be reviewed by the scientific committee.

Prepare your abstract as a word document using the following styles:

Title: Times New Roman 14pt bold

Authors: Times New Roman 12pt bold, presenting author underlined

Affiliations : Times New Roman 12pt, normal italic

One empty line

Text: Times New Roman 12pt normal, not more than 1 page total

Registration

The conference fee includes registration, all meals and accommodation from May 26 - 29, 2005. Sessions will start at 2pm Thursday, May 26 and will finish with dinner on Saturday, May 28. Your stay until Sunday is included in the registration.

Early registration until February 28th, 2005 

Students sharing double room

Late registration after March 1st, 2005

Bustransfer Vienna Airport/Semmering
(see Venue and Travel)
             one way
             return

540 Euro 

490 Euro 

720 Euro


 
  17 Euro
  34 Euro

To register please fill out the registration form (doc) and send to the Conference Secretary either by fax (+43 1 369 7615) or by email ( singlecell@boku.ac.at ).

Payment: Banktransfer to "Kongress" at  Österreichische Postsparkasse, Wien
BIC: OPSKATWW
IBAN: AT096000000092170553

Deadlines

Submission of Abstracts: December 18th, 2004

Extended Submission of Poster Abstracts: February 28th, 2005

Notification of Authors: January 31th, 2005

Early Registration until: February 28th, 2005

Grants

 
 
Students and Young Scientists up to the age of 36, who are either a member of FEMS or are recommended by a member, can apply for a Young Scientist Grant from FEMS to the Conference chair. Please use the form found at
http://www.fems-microbiology.org and send to the Organiser, not to FEMS! 

Submission deadline: February 28th, 2005.

Venue and Travel

The conference will take place at the Hotel Panhans in Semmering, Austria. Dating back to the late 19th century, when it accommodated Vienna`s High Society during their summer vacation, the Panhans combines Fin de Siecle charm with beautiful mountain scenery.

Semmering can be reached from Vienna´s South Train Station (Südbahnhof). Trains leave approximately every hour. Special Buses will run between Vienna Airport and Semmering on May 26th between approx. 10am and 1pm. On May 29th there´ll be transfers back to the airport at times to be fixed (depending on your needs). The bus service will cost 17€ one way or 34€ both ways. To book please add to your registration fee and inform us of your arrival and departure times at singlecell@boku.ac.at .

Scientific Committee

 

Nicole Borth, Austria (Chair)

Mhairi McIntyre, USA

Jeff A. Cole, UK

Susann Müller, Germany

Josep Gasol, Spain

Gerhard Nebe-von-Caron, UK

George Georgiou, USA

Tohoru Katsuragi, Japan

Diethard Mattanovich, Austria

Danilo Porro, Italy

Howard Shapiro, USA

Friedrich Srienc, USA

Organisation

Conference Secretariat "Single Cell Analysis"

Institute of Applied Microbiology, Department of Biotechnology

Universität für Bodenkultur

Muthgasse 18

1190 Vienna, Austria

Tel: +43 1 36006 6232

Fax +43 1 369 7615

Singlecell@boku.ac.at
 


 
On behalf of:

European Federation of Biotechnology

Section on "Microbial Physiology"

www.efbweb.org

http://www.tu-berlin.de/biotec/mibi/AG_Lang/efb/
   

We gratefully acknowledge Sponsorship by

Agilent

Austrian Federal Ministry for Education, Science and Arts

Austrian Federal Ministry for Economy and Work

Austrian Society of Biotechnology

Becton Dickinson Biosciences

Biorad

Dako Cytomation

Federation of European Microbiological Societies

Instrumentation Laboratory Beckman Coulter

Olympus

Partec

THP Medical Products

Zeiss