Progress in Pathogen-mediated Resistance Breeding against Plum Pox Virus

A. da Câmara Machado, E. Knapp, H. Pühringer, V. Hanzer, H. Weiss, Q. Wang, H. Katinger and Margit Laimer da Câmara Machado ; 1995

Institute of Applied Microbiology, University of Agriculture, Vienna/AUSTRIA
URL: http://www.boku.ac.at/iam iam@mail.boku.ac.at
Nussdorfer Lände 11, A-1190 Vienna, AUSTRIA (+43 1 3692924 - 402 FAX: 400 )

Abstract

As we already presented in the previous ISHS Fruit Tree Virus Meetings (Thessaloniki, Vienna), we are engaged in pathogen mediated resistance breeding to induce resistance in apricots and plums against plum pox virus (PPV) (Laimer et al., 1989, da Câmara Machado et al., 1992), PPV certainly being one of the most important pathogens of stone fruit trees in Europe and the whole Mediterranean area.

We have already reported the transformation and regeneration of Prunus armeniaca (Laimer da Câmara Machado et al., 1992) and P. domestica plants with Agrobacterium tumefaciens strain LBA 4404 containing various binary plasmids, pBinGUSint, carrying the marker gene ß-glucuronidase (GUS) and pBinPPVm, carrying the coat protein gene of plum pox virus (PPV).

Here we present our efforts to introduce different sequences from the viral genome of PPV into plants to induce resistance. Also new transformation protocols, which have been developed, allowing an efficient transfer of foreign genes into fruit trees, e.g. different apricot and plum cultivars, will be discussed.

Also our system and some results obtained with in vitro studies on the long term distribution of PPV will be presented. Encouraged by results of coat protein-mediated protection obtained with herbaceous hosts of PPV (da Câmara Machado et al. 1922, Regner et al. 1992) we conducted similar challenge infection experiments to investigate resistance phenomena with transgenic Prunus armeniaca and P. domestica. First results of these experiments will be presented.

Refrences

da Câmara Machado A., Regner F., Steinkellner H., Mattanovich D., Hanzer V., Weiss H., Laimer da Câmara Machado M., Katinger H. 1992. Coat protein-mediated protection against Plum Pox Virus. Acta Hort.309:203-210.

Laimer M., da Câmara Machado A., Hanzer V., Himmler G., Mattanovich D. and Katinger H.W.D. 1989. Regeneration of shoots from leaf discs of fruit trees as a tool for transformation. Acta Hort. 235, 85-92

Laimer da Câmara Machado M., da Câmara Machado A., Hanzer V., Weiß H., Regner F., Steinkellner H., Mattanovich D., Plail R., Knapp E., Kalthoff B., Katinger H. 1992. Regeneration of transgenic plants of Prunus armeniaca containing the coat protein gene of Plum Pox Virus. Plant Cell Reports 11(1): 25-29

Regner F., da Câmara Machado A., Laimer da Câmara Machado M., Steinkellner H., Mattanovich D., Hanzer V., Weiß H., Katinger H. 1992. Coat protein mediated resistance to Plum Pox Virus in Nicotiana clevelandii and benthamiana. Plant Cell Reports 11(1): 30-33

In: Acta Hort., in press

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04/13/95 C by IAM