Institut für Angewandte Mikrobiologie Institute of Applied Microbiology ------------------------------------------------------------------------------------------------------------------- Universität für Bodenkultur, Wien University of Natural Resources and Applied Life Sciences, Vienna, AUSTRIA Department of Biotechnology

Programm

Education

• Teaching Programme @ IAM

Cooperation

• ACBT – The Biotechnological Competence Centre in Austria

Methodical platforms

• Bioprozess Engineering
  • Characterisation of CHO Subclones Showing Profound Changes in Performance when Propagated in Different Cultivation Systems
• Quality Management
• Biopharmaceutical Drug delivery
  • A novel approach for antibiotic free selection of recombinant plasmids for gene therapeutic applications
    based on RNA antisense reaction
• Bioinformatics / Bioanalysis
  • Implementation of DNA-µ-array technology as a monitoring tool
    for recombinant E.coli processes
  • Improved monitoring of recombinant E.coli processes by advanced data exploration
    and chemometric modelling
  • Cross-Species Transcriptom Analysis of Recombinant Chinese Hamster Overy Cells
• Applied Immunology and Virology
  • Anti-idiotypic Antibodies, an alternative strategy against HIV-1
  • Influenza A virus as a vector for foreign antigens
  • Live Influenza Virus Vector as a Carrier for HIV-1 Antigens
  • Influenza A Virus Vector Expressing Interleukin-2 shows Increased Immune Response
    and Potential Antitumor Activity against Lung Cancer in a Murine Model
  • Intranasal Immunization with Live Attenuated Influenza Vectors Expressing ESAT-6 Antigen Protects Mice against Tuberculosis
  • Determining the humoral response to active immunization with monoclonal antibody against EpCAM
    using peptide arrays prepared by SPOT technology and yeast surface display system
  • Baculovirus - Vector Design for Gene Delivery in Mammalian Cells
  • Using baculovirus as a reverse genetics system for influenza B virus production
  • Development of a Novel Method for a Fast and Efficient Generation of Influenza Live Attenuated Vaccine by Co-Infection of Virus Like Particles
• Biodiversity Conservation and Utilisation
  • Biodiversity and Biodeteriorative Potential of Microcolonial Fungi isolated from Antique Marble Monuments in Antalya/Turkey
  • Identification of filamentous fungi and yeasts and their diversity in soils
    of the alluvial zone national park along the river Danube downstream of Vienna, Austria ("Nationalpark Donauauen")
  • Molecular Characterisation of Yeasts Isolated during spontaneous fermentation of Grape Must
• Cell and Molecularbiology
  • When cells get old: Identification and characterization of proteins involved in cellular aging
  • Aging and immortalization - tightly connected processes
  • Overexpression of SNEV correlates with enhanced DNA damage repair capacity in human endothelial cells
  • Characterization of the novel protein Nopsi (WBSCR20A)
  • Identification of BLOM7alpha, a putative RNA-binding protein, as androgen receptor and p21 coactivator
  • Does the novel protein Blom7 induce cell cycle arrest?
  • Analysis of changes in the protein expression profiles of HUVECs during replicative senescence reveals heterogeneity
  • Changes of the "ubiquitinome" during cellular ageing
  • Telomerase activity of medullary thyroid carcinoma cell lines is not sufficient for maintenance of telomere length,
    which eventually results in a period of "crisis"
  • Intracellular H2O2 level declines with age in renal tubular epithelial cells
  • Establishment of a continuously growing fibroblast cell line derived from a Williams-Beuren syndrome patient
  • Improved Antisense-constructs for targeted gene-silencing mediated by ColE1-type plasmid attendance

Technology platforms

• Cell factories
  • Flow Cytometry and Cell Sorting as Tools to understand and optimize Cell Factories
  • Sorting for High Producers: Methods and Applications
  • From Gene to Antibody: Efficient Screening by Cell Sorting
  • Improvement of the glycolytic metabolism in mammalian cells by fluorescence activated cell sorting
  • High throughput methods for strain improvement - Microarrays
  • High throughput methods for strain improvement - Flow Cytometry & FACS
  • Improvement of lactic acid production in yeast by cell sorting for high intracellular pH
  • Optimisation of protein secretion in antibody fragment expressing Pichia pastoris
• Biopharmaceutical Technology
  • Protein Biopharmaceuticals from Mammalian Cell Technology: from Gene to the Master Cell Bank
  • Monitoring of Glycoprotein Quality by Differential Isoelectric focusing
• Liposomology
  • GMP Production of Liposomen - a new industrial approach
  • Efficacy of topically applied liposomal encapsulated superoxide dismutase (SOD) in experimental scalds - a controlled animal study
  • Experimental Design for in-vitro penetration study of liposomal superoxide dismutase (SOD)
• Protein Technology
  • Selection and characterization of phage-display scFvs directed against Ep-CAM
  • Cloning, Expression, Purification and Characterization of human Alpha-Fetoprotein
  • Novel high capacity and alkaline stable Protein A media
  • Screening of Aryl hydrocarbon receptor ligands in yeast
  • Green fluorescent protein as reporter for transactivation assays
• Plant Biotechnology
  • Biotechnology of fruit trees @ IAM
  • Molecular characterisation of transgenic grapevines carrying sequences of a Nepovirus coat protein gene
  • Regeneration and molecular characterisation of transgenic stone fruit (Prunus sp.)
  • Simple sequence repeat (SSR) analysis for assessment of genetic variability in apricot cultivars
  • In vitro sanitation of an old Croatian grapevine cultivar
  • Localisation and distribution of the major allergens in apple fruits and tissues
  • Novel allergen of raspberry is cross-reactive with homologues in birch pollen and apple fruits
  • more links: Plantbiotechnology group @ IAM
• Downstreamprocessing
  • Quantitative Evaluation of Proteins binding to in situ synthesized Peptides: Preparatory Analysis for Microbiochips
  • Thermodynamic stability and formation of aggregates of human IgG during processing
  • Adsorption of plasmid DNA on rigid superporous anion exchange adsorbent matrix
  • In-situ determination of adsorption kinetics of proteins in a finite bath
• Animal Cell Technology
  • Stable Processes for Safe Products
  • Protein Free Transfection of CHO Host Cells with an IgG-Fusion Protein - Selection and Characterisation of Stable High Producers
  • Implementation of an Automated Medium Feed Control System for Perfusion based Fermentation Processes
  • SOFTMIXER®, a New Scalable Mixing System for Protein-free Cultivation of Shear-Sensitive Cells on Microcarrier
• Microbial Technology
  • Microbial Fermentation Group - overview
  • In-depth Momitoring of recombinant E.coli cultivations
  • Modulation of Inclusion Body (IB) Formation Kinetics
    by Different Induction Regimes in E.coli Fed Batch Cultivations
  • Investigation of inclusion body formation kinetics by FTIR-spectroscopy
  • Monitoring of stress response during recombinant protein production in E.coli
  • Fermentation and Microbial Additives
  • Biological Inactivation of Trichothecenes - Industrial/Academic Co-operation
  • Trichosporon mycotoxinivorans sp. nov., A new yeast species useful in biological detoxification of various mycotoxins
• Environmental Biotechnology
  • A Fuzzy Logic Approach to Control Anaerobic Digestion
  • Microbial Fuel Cells for Direct Energy Production
  • CROPGEN - Renewable energy from crops and agro-wastes
  • Environmental Biotechnology at IFA-Tulln
  • Microbiological Ecology
  • Anaerobic Digestion
  • Water/Wastewater Treatment
  • Contaminated Land Manegement