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Research

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CV

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Current Address	Igeneon Immunotherapy of Cancer Brunner Strasse 69/3 A-1230 Vienna Tel: +43-1-90250-202
University Address:	Institute for Applied Microbiology (IAM) University for Agricultural Sciences  Muthgasse 18  A-1190 Vienna  Austria  Follow this link to get directions to the Institute
Research Areas:	Bioprocess Engineering and Bioprocesscontrol Large Scale Cellculture and Bacterial Fermentation Production of clinical grade biologics Biosafety
Research Projects:	Ultrasound Cell Separation System Principles of ultrasonic cell separation EU funded Training and Mobility Program (TMR)  Financing: FFF (Partner: Anton Paar KG., Inst. f allgemeine Physik)    Large Scale Production of Clinical Grade Biologics Overwiew of the IAM Pilot Plant Fluidized bed technology Clinical grade monoclonal antibodies Financing: Various Industrial Cooperations  Biological Safety The integrated biosafety approach, Engineering and Validation of L3 Laboratories Insect Cells: Expressionsystem for Recombinant Proteins Financing: FWF (Fonds zur Foerderung der wissenschaftlichen Forschung)  Bioprocess Control Industrial process control systems Strategies for optimal dissolved oxygen (DO) control Financing: Industrial Cooperations: Vogelbusch, Honeywell, Elin  Fuzzy Control Strategies in Bioprocess Control  Financing: Industrial Cooperation: Omron  Biosensors for the Optimization Bioprocesses Financing: FFF ( Partner: Bender GesmbH., Inst. f. allg. Biochemie UNI Wien)
Affiliations:	Expert for the Austrian gentechnology commission  Member of the board Österreichische Gesellschaft für Biotechnologie (ÖGBT)  Member European Biosafety Association (EBSA) Member of the New York Academy of Science Consulting Editor Cytotechnology International Editor Applied Biosafety  Chairman of the Working Party "Safety in Biotechnolgy" of the European Federation Biotechnology (EFB) from  1996 to 2002  Member and editor in the Experts Group "Safety in Biotechnology" of the International Society for Social Security (IVSS)  - 2000  Member of the Organizing Team for the EU concerted action Safety Assessment of Biotechnological Processes and Products in the Agro-Food Area (SABAF)  - 1998
CV	1979-1985:  University of Agricultural Sciences, Vienna, Austria,                      Spec. Food and Biotechnology                      Summer jobs:                      ) Austrian Sugar Research Institute                      ) Tate and Lyle Research Dep., Reading U.K.                      ) Diary, Quality Control  1983-1984:  Projectwork at the Inst. for Appl. Microbiology Univ.                      for Agric., Vienna                      Research abroad: INSA Toulouse, Prof. G. Goma                      Mastersthesis: Optimizing parameters for alpha-Amylases                      production by Aspergillus Oryzae  1985     :      Diplom Ingenieur (equiv. Masters)  1985-1987:  Continued Project work at the IAM, Vienna                      Research Assistant                      Thesis: Processcontrol in Biotechnology                      Research abroad:                      ) Biosensors for fermentation control, research at the                      Cranfield Biotechnology Center, U.K.  1987     :      PhD  1987-1988:  Continued Projectwork at the IAM:                      ) On-line ex-situ ion selective microsensor for                      fermentation processes                      ) Engineering and layout of a fermentation pilot plant                      at the IAM (process control)  1988     :       Consulting and engineering for Austrian Biotechnology                      Consultants at:                      AMGen Inc., Thousand Oaks, California, USA  1988-1989:  Schrödingergrant from the Austrian Science Foundation,                      Researchwork in the fields of Biosensors and                      Bioanalytics                      ) University of Delaware, Newark, Delaware, USA, ept.                      Chem. and Biochem. (Prof. Rechnitz)                      ) Biotechnology Research Institute, Montreal, Canada  since May 1989      Projectwork at the IAM                      ) Design, installation and operation of IAM Pilotplant                      ) Processcontrol and Scale-up of biotechnological                      Production Processes  1989 - 1996    Head of the IAM GMP pilot plant  Since February      University Assistant Professor at the IAM  1990:                     Research Areas:                      ) Biosafety in Biotechnology                      ) Large Scale-Production of biologics for clinical                      testing, diagnostics and affinity purification                      ) Cell retention devices for animal cell culture                      ) Novel Control Strategies in Bioprocess control (e.g.                      fuzzy control)                      ) Biosensors for Fermentation Control                      ) Insect cell culture                      ) Baculovirus Display Systems  Since June 1994:    Associate Professor at the IAM  Dec. 1994    Collab. Research at Hiroshima City University (Prof. T.Takeuchi)  - Feb 1995:  ) Molecular Imprinting    Feb 1996:     Collab. Research at Hiroshima City University (Prof. T. Takeuchi)                      ) Molecular Imprinting  Since 1992:   Biosafety consultant to the Austrian gene technology regulatory authorities Since 1999:   GMP consultant to the industry 2001 - 2002: Coordinator of the Austrian Center for Biopharmaceutical Technology Since 2002:   Head Production and Head Technical Affairs, Igeneon Since 2005:    Vice President Manufacturing, Apthon Corporation
Further Qualifications:	1996:               International COMETT Course on "Biological Safety in Industrial and Agricultural Biotechnology" 1996:               Course on Biosafety of Transgenic Organisms in Human Health Products, FIOCRUZ, UNIDO, Rio de Janeiro, Brazil 1998:               Second Workshop of Experts on Biosafety, Rio de Janeiro, Brasil 1999:               EU Advanced Workshop on Biosafety under participation of the EFB Working Group Safety in Biotechnology  2002:               Course on Advanced Good Manufacturing Practices for Biotechnology Products, Leiden, Netherlands 2002:               Well Characterized Biologicals 2002:               European Compliance Academy, GMP Education Course, EU-GMP and FDA Compliance in Pharmaceutical Development, Madrid, Spain 2003:                Product Characterization of Antibodies, Copenhagen, Denmark 2004:               Certified Auditor ICH Q7A, Compliance for APIs manufactured by cell culture and fermentation
Publications:	K. Bayer, O. Doblhoff-Dier, Third European Congress on Biotechnology, Munich 1984, Verlag Chemie, Vol I, p 725-730, "Optimizing fungal amylase production in submerged culture"  O. Doblhoff-Dier, K. Bayer, H.W.D. Katinger, P. Svasek, J. Jansky, F. Paschke,  "A modular microsensor for continous monitoring of fermentation processes", in: Developments in Biological Standardisation, vol 66, pp 345-348, 1987  O. Doblhoff-Dier, K. Bayer, P. Svask, J. Jansky, 1st IFAC Symposium on Modelling and Control of Biotechnolocal Processes, 1985 "Modular and highly flexible measurement and control system for bioprocess developement"  O. Doblhoff-Dier, K. Bayer, P. Svasek, H. Jansky, Biotech-Forum 3 (1986) pp 219-233, "Modular and Highly Flexible Measurement and Control System for Bioprocess Developement"  O. Doblhoff-Dier, K. Bayer, H. Katinger, F. Kohl, P. Svasek, J. Jansky, F. Paschke, Proc. "4th European Congress on Biotechnology, Amsterdam, 1987, "Modular Hard and Software Concept for Labscale Computercontrolled Fermentation"  O. Doblhoff-Dier, K. Bayer, H. Katinger, Informationsveranstaltung des Bundesministeriums. Wiss. Forschung, Bad Ischl 1987, "Einrichtung einer computergestützten Laboranlage zur Züchtung tierischer Zellen"  W. Kramer, O. Doblhoff, K. Bayer, 4. Int. Workshop (Microb. Physiol. and the Manufact. Industry, Hadjuszobozlo (Hungary), 1988, "Kinetic Studies of Bacterial alpha Amylase Production"  M. Reiter, P. Schwengler, W. Ernst, F. Weigang, F. Steindl, O. Doblhoff-Dier, N. Borth, HWD Katinger, Abstracts Engineering Foundaton Conference on Cell Culture Engineerinig, Palm Coast, Florida, USA. (1988), "Physiological Patterns of cultured Hybridomas: Metabolic Flux Rates of Amino Acids and Substrates of the Glycolytic Chain"  HWD Katinger, M. Reiter, F. Weigang, W. Ernst, O. Doblhoff-Dier, N. Borth and F. Steindl, Abstracts Engineering Foundation Conference on Cell Culture Engieering, Palm Coast, Florida, USA (1988), " A Scalable Modular Vibrating Cage System for Enhanced Gas/Liquid Diffusion and Cell Immobilisation in Continous Perfused Culture of Mammalian Cells"  O. Doblhoff-Dier, P. Schwengler, B. Ehrenhuber, W. Kramer, K. Bayer, H. Jansky, P. Svasek, F. Kohl, Biotechnology and Bioengineering, 34 (10), 1326, (1989), " On-line Monitoring of NH4 Concentration with Compendation of Interfering Ion Species Influence by Simultaneous Determination of Na+ and K+ with Modular Microscale Ion Selective Fluid Membrane Sensors."  O. Doblhoff-Dier, G.A. Rechnitz, Analytical Letters 22, 1047, (1989), "Amperometric Enzyme Based Biosensor for the Detection of Xanthine Via Superoxide"  O. Doblhoff-Dier, G.A. Rechnitz, Analytica Chimica Acta 222, 247-252, (1989), "Amperometric Method for the Determination of Superoxide Dismutase Activity at Physiological pH"  O. Doblhoff-Dier, S. Huss, R. Plail, F. Unterluggauer, W. Kramer, N. Taferner, K. Bayer, H. Katinger, 5th European Congress on Biotechnology Copenhagen, Munksgaard, 179, (1990), "Pilot Plant Production of Recombinant Proteins"  W. Kramer, O. Doblhoff-Dier, H.P. Lettner, D. Pettauer, N. Taferner, K. Bayer, H. Katinger, 5th European Congress on Biotechnology Copenhagen, Munksgaard, 345, (1990), "Developement of a Control Strategy for Production of Recombinat Proteins  Huss Siegfried,Karl Bayer,Otto Doblhoff-Dier,Walter Kramer,Robert Lithos,Regina Plail,Florian Unterluggauer,Hermann Katinger,SYNOPSIS 1989, Eigenverlag Institut f.Angewandte Mikrobiologie, "BIOTECHNIKUM"  Karl Bayer,Otto Doblhoff-Dier,Siegfried Huss,Walter Kramer,Hans Peter Lettner,Robert Lithos,Regina Plail,Judith Salzmann,Norbert Taferner,Florian Unterluggauer,Bernd Winkler, SYNOPSIS 1989, Eigenverlag Inst.f.Angewandte Mikrobiologie, "BIOPROZESSKONTROLLE"  Otto Doblhoff-Dier, Regina Plail, Manfred Reiter, "Laborsicherheit", in: Gen- und Biotechnologie, Österr. Umweltbundesamt, 1991  O. Doblhoff-Dier, S. Huss, R. Litos, R. Plail, F. Unterluggauer, M. Reiter, H.W.D. Katinger, Process Biochemistry, 26, 201-207, (1991) "A Modular Computer Controlled Fermentation Pilot Plant"  M. Reiter, G. Blüml, T. Gaida, N. Zach, F. Unterluggauer, O. Doblhoff-Dier, M. Noe, R. Plail, S. Huss and H. Katinger, Bio/Technology, 9, 1100-1102, (1991) "Modular Integrated Fluidized Bed Bioreactor Technology"  O. Doblhoff-Dier, F. Unterluggauer, S. Huss, R. Plail, H.W.D. Katinger, "Pilot Scale Modular Hard and Software Concept For Animal Cell Fermentation", in:_ Animal Cells: Developments, Processes and Products (Spier, Griffiths and MacDonald, eds.) Butterworth-Heinemann Ltd., Oxford, UK, 393-396, (1992)  M. Reiter, N. Zach, T. Gaida, G. Blüml, O. Doblhoff-Dier, F. Unterluggauer and H. Katinger, "Oxygenation in Fluidized Bed Bioreactors using the Microsparging Technique", in: Animal Cells: Developments, Processes and Products (Spier, Griffiths and MacDonald, eds.) Butterworth-Heinemann Ltd., Oxford, UK, 386-390, (1992)  F. Unterluggauer, O. Doblhoff-Dier, M. Reiter, G. Blüml, N. Zach, T. Gaida, G. Kral, C. Schmatz and H. Katinger, "Scale-up of a Fluidized Bed Reactor Operated  with Porous Glass Carriers", in: Animal Cells: Developments, Processes and Products (Spier, Griffiths and MacDonald, eds.) Butterworth-Heinemann Ltd., Oxford, UK, 505-507, (1992)  K. Bayer, O. Doblhoff-Dier, H. Katinger, A.P. Loibner, M. Reiter, N. Zach, E. Mann-Buxbaum, I. Moser, F. Pittner, Th. Schalkhammer, AnaBiotec 1992, "Application of a FIA Biosensor System in Fermentation Control"  W. Frommer, L. Archer, B. Boon, G. Brunius, C.H. Collins, P. Crooy, O. Doblhoff-Dier, R. Donikian, J. Economidis, C. Frontali, T. Gaal, S. Hamp, H. Haymerle, E.H. Houwink, M.T. Küenzi, P. Krämer, H.L.M. Lelieveld, M.Th. Logtenberg, J. Lupker, S. Lund, J.L. Mahler, C. Mosgaard, F. Normand-Plessier, F. Rudan, R. Simon, G. Tuijnenburg Mujs, S. Vranch, R. Werner, "Safe biotechnology (5). Recommendations for safe work with animal and human cell cultures concerning potential human pathogens", Applied Microbiology and Biotechnology, 39, pp 141-147, (1993)  F. Unterluggauer, O. Doblhoff-Dier, C. Tauer, A. Jungbauer, T. Gaida, M. Reiter, C. Schmatz, N. Zach and H. Katinger, "Stable contionuous large scale production of human monoclonal HIV-1 Antibody using a computer controlled pilot plant", BioTechniques, 16,1, pp 140-147, (1994)  E. Benes, W. Burger, M. Gröschl, A. Schaffner, F. Trampler, W. Bolek, T. Gaida, O. Doblhoff, F. Hager, "Trapping of suspended biological particles by use of ultrasonic resonance fields". In: Ultrasonics International '93, Conference Proceedings, Butterworth-Heinemann, Oxford, UK [1993], pp. 515-518 (ISBN 0 750618 779). A.P. Loibner, O. Doblhoff-Dier, N. Zach, K. Bayer, H. Kattinger, Ch. Lobmaier, Th. Schalkhammer, F. Pittner, Automated Glucose Measurement with Micro Structured Thin Layer Biosensors for the Control of Fermentation Processes, Eurosensors VII, Budapest (1993) A. Loibner, O. Doblhoff-Dier, N. Zach, K. Bayer, "Automated Glucose Measurement with Micro Structured Thin Layer Biosensors for the Control of Fermentation Processes" in Sensors&Actuators B,18-19,603-606, 1994   O. Doblhoff-Dier, K. Bayer, A. Jungbauer, "Monitoring and Control", in: Recovery of Bioproducts, G. Schmidt-Kastner, D. Sell, K. Biedermann, M.P. Brocklebank, A. Jungbauer and J.A. Tijsterman Eds., SCI London, 1993  O. Doblhoff-Dier, K. Bayer, "Computer controlled fermentation", in:Protocols in Cell and Tissue Culture, J. Griffiths, A. Doyle, G. Newell Eds., John Wiley and Sons, Chichester O. Doblhoff, Th. Gaida, H. Katinger, W. Burger, M. Gröschl, E. Benes, "A novel ultrasonic resonance Field Device for the Retention of Animal Cells", Biotechnology Progress, 10, 428-432, (1994)  B. Jank, O. Doblhoff-Dier, J.v.d.Plas, B. Habermann, “Sequence motifs in a flagellin of Pseudomonas putida“, Microbiology 141, (1995)  H.L.M. Lelieveld, H. Bachmayer, B. Boon, G. Brunius, K. Bürki, A. Chmiel, C.H. Collins, P. Crooy, O. Doblhoff-Dier, I. Economidis, A. Elmquist, W. Frommer, C. Frontali-Botti, R. Havenaar, H. Haymerle, C. Hussey, O. Käppeli, M. Lex, S. Lund, J.L. Mahler, R. Marris, Ch. Mosgaard, C. Normand-Plessier, F. Rudan, R. Simon, M.Th. Logtenberg, R.G. Werner, “Safe Biotechnology. Part 6 Safety assessment, in respect to human health, of microorganisms used in biotechnology“ ,  Applied Microbiology and Biotechnology 43: 389-393 (1995)  Th. Gaida, O. Doblhoff-Dier, K. Strutzenberger, H. Katinger,W. Burger, B. Handl, M. Gröschl, E. Benes, “Selective retention of viable cells in ultrasonic resonance field devices“, Biotechnology Progress, 12, p 73-76  , (1996)  J. Matsui, O. Doblhoff-Dier, T. Takeuchi, „Atrazine-selective Polymer Prepared by Molecular Imprinting Technique“, Chemistry Letters, p 489, (1995)  Th. Gaida, O. Doblhoff, H. Katinger, W. Burger, M. Gröschl, E. Benes, "Scale-up of ultrasonic resonance field cell separation devices used in animal cell technology", in: Animal Cell Technology: Developments towards the 21st Century, E.C. Beuvery, J.B. Griffiths, W.P. Zeijlemaker (eds.), Kluwer, Dordrecht, p 699-703 (1995)  O. Doblhoff-Dier, T. Gaida, W. Burger, B. Handl, M. Gröschl, E. Benes, "Abscheidung dispergierter Teilchen durch Ultraschall-induzierte Koagulation: Anwendung in der tierischen Zellkultur". In: Berichte der Informationstagung Mikroelektronik 1995, ÖVE, Wien [1995], pp. 385-388 (ISBN 3-85133-003-X). B. Jank, A. Haslberger, O. Doblhoff-Dier  Sicherheitseinstufung gentechnischer Arbeiten in geschlossenen Systemen. Lebensmittel- & Biotechnologie 4:140-141, (1995)  J. Matsui, Y. Miyoshi, O. Doblhoff-Dier, T. Takeuchi, „A Molecularly Imprinted Synthetic Polymer Receptor Selective for Atrazine" Analytical Chemistry 67:4404-4408, (1995)  H.L.M. Lelieveld, H. Bachmayer, B. Boon, A. Bennett. G. Brunius, A. Chmiel, C.H. Collins, P. Crooy, O. Doblhoff-Dier, I. Economidis, A. Elmquist, C. Frontali-Botti, R. Havenaar, H. Haymerle, O. Käppeli, G. Leaver, M. Lex, S. Lund, J.L. Mahler, R. Marris, J.L Martinez, Ch. Mosgaard, C. Normand-Plessier, M. Romantschuk, F. Rudan, K. Wagner, R.G. Werner, “Safe Biotechnology. Part 7. Classification of microorganisms on the basis of hazard" Applied Microbiology and Biotechnology, 45:723-729, (1996)  B. Jank, H. Haymerle, O. Doblhoff-Dier, „Process Biosafety: Zürich hazard analysis in biotechnology. Can risk managment be improved using proactive communication strategies?“, Bio/Technology 14:894-896, (1996)  M. Gröschl, E. Benes, B. Handl, O. Doblhoff-Dier, T. Gaida, "Ultrasonic resonators for particle separation", IEICE Tech. Report 96 (432) [1996], pp. 1-6. O. Doblhoff-Dier, „Cell lines: Applications and Biosafety“, in: Biosafety of Transgenic Organisms in Human Health Products, L. Macedo Oda (Ed.), pp. 91-106, (1996)  O. Doblhoff-Dier, R. Bliem, „Assuring quality of biopharmaceuticals: A prerequisite to start business and stay in business“, BINAS News: Vol. 2, Issue 2, 1996  Available on-line: http://binas.unido.or.at/binas/News/96issue2.html#ass    H. Katinger, A. Assadian, G. Blüml, N. Borth, A. Buchacher, O. Doblhoff, T. Gaida, M. Reiter, C. Schmatz, K. Strutzenberger, W. Steinfellner, F. Unterluggauer, N. Zach, "Long-term stability of continuously perfused animal cells immobilized on novel macroporous microcarriers", in: Advances in Molecular and cell Biology, Biochemical Technology, Vol. 15A, E.E. Bittar, B. Danielsson, L. Bülow (Eds.), pp. 193-208, (1996)  R. Grabherr, W. Ernst, O. Doblhoff-Dier, M. Sara, H. Katinger, "Expression of Foreign Proteins on the Surface of Autographa Californica Nuclear Polyhedrosis Virus", Biotechniques 22, pp 730-735, (1997)  J. Matsui, O. Doblhoff-Dier, T. Takeuchi, "2-(Trifluormethyl)acryl acid: a novel functional monomer in non-covalent molecular imprinting", Analytica Chimica Acta 343, 1-4, (1997)  M. Gröschl, W. Burger, B. Handl, O. Doblhoff-Dier, T. Gaida, C. Schmatz, "Ultrasonic separation of suspended particles - Part III: Application in biotechnology", Acustica - acta acustica 84 (5) pp. 815-822 (1998) O. Doblhoff-Dier, " Implications of a strengthened biological and toxin weapons convention for the biotechnology R&D", Proceedings of the conference on: A Strengthened Biological and Toxin Weapons Convention (BTWC): Potential Implications for Biotechnology, 28. - 29. May 1998, Institute of Applied Microbiology, Vienna, Austria,  Also available on-line: http://www.boku.ac.at/iam/efb/btwcabst.doc O. Doblhoff-Dier, " Maintaining awareness of developments in biosafety regulations and controls ", Proceedings of the conference on: A Strengthened Biological and Toxin Weapons Convention (BTWC): Potential Implications for Biotechnology, 28. - 29. May 1998, Institute of Applied Microbiology, Vienna, Austria  Also available on-line: http://www.boku.ac.at/iam/efb/btwcabst.doc O. Doblhoff-Dier, R. Bliem " Quality control and quality assurance: Essentials from development to production of biopharmaceuticals", Trends in Biotechnology, 17, 7, 266-270 J. Rath, B. Jank, O. Doblhoff-Dier, " Biological Weapons Control", Science,p 2194, (1998)
	O. Doblhoff-Dier, Bliem R. Quality control and assurance from the development to the production of biopharmaceuticals, Trends Biotechnol 1999 Jul 17:7 266-70 B. Jank, O. Doblhoff-Dier, "Gen- und Biotechnologie - Die Grundlagen einer sicheren Anwendung", Umweltschutz, 42-44, (1999) Doblhoff-Dier O. et al., The WP Safety in Biotechnology of the European Federation, Biotechnology, Safe Biotechnology. Part 9. Values in risk assessment for environmental application of microorganisms, Trends in Biotechnology, 1999, 17, 307 - 311  Johannes Rath, Bernhard Jank, Otto Doblhoff-Dier, "Confidentiality is vital to bioweapons control", Nature 401, 424 (1999) Jank, B., A. Berthold, S. Alber, and O. Doblhoff-Dier, "Assessing the Impacts of gentically modified microorganisms". Int J LCA, 1999. 4(5): p. 251-252  O. Doblhoff-Dier, T. Gaida, W. Burger, F. Trampler*, M. Gröschl, E. Benes, J.M. Piret, S.M. Woodside, Review on the viability of mammalian cells in ultrasonic standing wave fields, Acustica - acta acustica 85 (1999) Supplement 1, pp S399 - S400, as well as in Journal of Acoustical Society of America Vol. 105, p 1325 - 1326, February 1999 S. Radel*, A.J. McLoughlin, L. Gherardini*, O. Doblhoff-DIER, E. Benes, Viability of yeast cells in well controlled propagating and standing ultrasonic plane waves, Ultrasonics 38 (2000) 633-637 S. Radel*, A.J. McLoughlin, L. Gherardini*, O. Doblhoff-Dier, C.O. Connell, B. Cregg, D.C. Cottell, E. Benes , Morphology and viability of Saccharomyces cerevisiae in suspension stressed by ultrasonic plane waves, Proc. of the 23rd Annual Symposium of the Microscopical Society of Ireland, Belfast, August 31st - September 2nd, 1999 S. Radel*, A.J. McLoughlin, L. Gherardini*, O. Doblhoff and E. Benes, Morphology and viability of suspended yeast cells stressed by ultrasonic plane waves Proc. 1. Öst. Workshop über Apparative Qualitätssicherung im bildgebenden medizinischen Ultraschall, Institut für Biomedizinische Technik & Physik, Universität Wien im AKH Wien (26.11.1999) 28 S. Radel*, A.J. McLoughlin, P. Walsh, L. Gherardini*, O. Doblhoff-Dier, E. Benes, Application of low intensity ultrasonic wave fields to manipulate and direct the immobilisation/bio-encapsulation of yeast cells, Proc. of the Eight International Workshop on Bioencapsulation, Trondheim, Norway, September 13th - 15th, 1999, CD-Rom: paper No. O-7 L. Gherardini*, S. Radel, A.J. McLoughlin, O. Doblhoff-Dier and E. Benes, A study of the spatial organisation of microbial cells in a gel matrix subjected to treatment with ultrasound standing waves, Joint Meeting COST Action 840 and EUSS TMR Network: Bioencapsulation Innovation and Technologies, University of Vienna, Center of Pharmacy (18. - 20.5.2000), Book of Abstracts, p 22 S. Radel*, A.J. McLoughlin, L. Gherardini*, O. Doblhoff-Dier and E. Benes, The mechanisms of morphology changes of yeast suspended in watery liquids and water-rich ethanol mixtures exposed to ultrasonic plane waves, Joint Meeting COST Action 840 and EUSS TMR Network: Bioencapsulation Innovation and Technologies, University of Vienna, Center of Pharmacy (18. - 20.5.2000), Book of Abstracts, p 37 S. Radel*, A.J. McLoughlin, L. Gherardini*, O. Doblhoff-Dier and E. Benes, Application of low intensity ultrasonic wave fields to manipulate and direct the immobilisation/bioencapsulation of yeast cells, Joint Meeting COST Action 840 and EUSS TMR Network: Bioencapsulation Innovation and Technologies, University of Vienna, Center of Pharmacy (18. - 20.5.2000), Book of Abstracts, p 38 L. Gherardini*, O. Doblhoff-Dier, E. Benes, S. Radel* and A.J. McLoughlin, Yeast cells immobilised in a gel matrix by ultrasound quasi standing waves: a new technique for the study of the spatial re-arrangement, Biotechnology 2000 - The World Congress on Biotechnology, Berlin/DE (3. - 8.9.2000), Book of Abstracts, Vol. 4, p 324 S. Radel*, A.J. McLoughlin, L. Gherardini*, O. Doblhoff-Dier and E. Benes, Non-lethal changes of yeast suspensions irradiated in a standing ultrasonic wave field generated by a cell retention system, Biotechnology 2000 - The World Congress on Biotechnology, Berlin/DE (3. - 8.9.2000), Book of Abstracts, Vol. 4, p 357 S. Radel*, A.J. McLoughlin, L. Gherardini*, O. Doblhoff-Dier and E. Benes, Application of low intensity ultrasonic wave fields to manipulate and direct the immobilisation/bioencapsulation of yeast cells, 30th Annual Food Science & Technology Research Conference, University College Cork/Irland (19. - 20.9.2000), Book of Abstracts, p 139 L. Gherardini*, S. Radel*, S. Sielemann*, O. Doblhoff-Dier, M. Gröschl, E. Benes and A.J. McLoughlin, A study of the spatial organisation of microbial cells in a gel matrix subjected to treatment with ultrasound standing waves, Bioseparation - Int. J. of Separation Science in Biotechnology, in print L. Gherardini*, O. Doblhoff-Dier, E. Benes, S. Radel*, A. J. McLoughlin, The spatial organisation of microbial cells in a gel matrix subjected to treatment with ultrasound standing waves, Proc. of the 24th Annual Symposium of the Microscopical Society of Ireland, Dublin, 10-12 Sept 2000, p 22 Safe biotechnology 10: DNA content of biotechnological process waste. European Federation of Biotechnology Safety in Biotechnology Working Party, Trends Biotechnol 2000 Apr 18:4 141-6 Chippeaux, A., Collins, C., Doblhoff-Dier, O., Le Bacle, C., Leprince, A., Ott, R.J., Püringer, Günther, E., Skrobanek, B., Adelmann, S., W., Pürro, S., Stalpers, J.A. ,Thoinet, M. & Truscheit, E. (1999). - Control of risks in Work with Biological agents Biotechnology, Genetic Engineering, Part 2, Laboratory Work, International Social Security Associaton ISSA, Series 2037- this series of booklets is available in English, German and French version from the ISSA, order from http://www.issa.int/engl/homef.htm S., Chippeaux, A., Collins, C., Doblhoff-Dier, O., Le Bacle, C., Leprince, A., Ott, R.J., Püringer, Günther, E., Skrobanek, B., Adelmann, W., Pürro, S., Stalpers, J.A. ,Thoinet, M. & Truscheit, E. (1999). - Control of risks in Work with Biological agents Biotechnology, Genetic Engineering, Part 2, Laboratory Work, International Social Security Associaton ISSA, Series 2038, ISBN 92-843-1138 - this series of booklets is available in English, German and French version from the ISSA order from http://www.issa.int/engl/homef.htm Günther, E., Skrobanek, B., Adelmann, S., Chippeaux, A., Collins, C., Doblhoff-Dier, O., Le Bacle, C., Leprince, A., Ott, R.J., Püringer, W., Pürro, S., Stalpers, J.A. ,Thoinet, M. & Truscheit, E. (1999). - Control of risks in Work with Biological agents Biotechnology, Genetic Engineering, Part 3, Production, International Social Security Associaton ISSA, Series 2039- ISBN No. 92-843-1139-X, this series of booklets is available in English, German and French version from the ISSA order from http://www.issa.int/engl/homef.htm Doblhoff-Dier, O. and G. Stacey (2000). Cell lines: Applications and Biosafety. Biological Safety - Principles and Practices. D. O. Fleming and D. L. Hunt. Washington, ASM Press: 221-241. View contents on-line M. Dürrschmid, K. Landauer, G. Simic, H. Klug, D. Müller, T. Keijzer, F. Trampler°, A. Oudshoorn°, M. Gröschl*, O. Doblhoff-Dier, Comparison of Fluidised Bed and Ultrasonic Cell-Retention Systems for High Cell Density Mammalian Cell Culture, ESACT 2001 - The 17th Meeting: From Target to Market, JUNE 10-14, 2001 - TYLÖSAND - SWEDEN O. Doblhoff-Dier, Collins CH, Biosafety: future priorities for research in health care, J Biotechnol 2001, 85:2, 227-39 Th Bachinger, C -F Mandenius, G Striedner, F Clementschitsch, E Dürrschmid, M Cserjan-Puschmann, O Doblhoff-Dier, K Bayer, Non-invasive detection of the metabolic burden on recombinant microorganisms during fermentation processes, Journal of Chemical Technology & Biotechnology 2001, 76:8 885-889 View on-line Radel S, Gherardini L, McLoughlin AJ, Doblhoff-Dier O, Benes E., Breakdown of immobilisation/separation and morphology changes of yeast suspended in water-rich ethanol mixtures exposed to ultrasonic plane standing waves. Bioseparation. 2000;9(6):369-77 Radel S, McLoughlin AJ, Gherardini L, Doblhoff-Dier O, Benes E. Viability of yeast cells in well controlled propagating and standing ultrasonic plane waves. Ultrasonics. 2000 Mar;38(1-8):633-7. Gherardini L, Radel S, Sielemann S, Doblhoff-Dier O, Groschl M, Benes E, McLoughlin AJ. A study of the spatial organisation of microbial cells in a gel matrix subjected to treatment with ultrasound standing waves. Bioseparation. 2001;10(4-5):153-62. Landauer K, Wiederkum S, Durrschmid M, Klug H, Simic G, Bluml G, Doblhoff-Dier O. Influence of carboxymethyl dextran and ferric citrate on the adhesion of CHO cells on microcarriers. Biotechnol Prog. 2003 Jan-Feb;19(1):21-9. Durrschmid MP, Landauer K, Simic G, Klug H, Keijzer T, Trampler F, Oudshoorn A, Groschl M, Muller D, Doblhoff-Dier O. Comparison of fluidized bed and ultrasonic cell-retention systems for high cell density mammalian cell culture. Biotechnol Prog. 2003 May-Jun;19(3):1045-8 Durrschmid M, Landauer K, Simic G, Bluml G, Doblhoff-Dier O. Scalable inoculation strategies for microcarrier-based animal cell bioprocesses. Biotechnol Bioeng. 2003 Sep 20;83(6):681-6. Rath J, Jank B, Doblhoff-Dier O. Journals must cooperate to defend biosecurity. Nature. 2004 Dec 2;432(7017):549.
Oral Presentations:	O. Doblhoff-Dier, K. Bayer, H. Katinger, F. Kohl, P. Svasek, J. Jansky, F. Paschke, Proc. "4th European Congress on Biotechnology, Amsterdam, 1987, "Modular Hard and Software Concept for Labscale Computercontrolled Fermentation", oral presentation  O. Doblhoff-Dier, K. Bayer, H. Katinger, F. Kohl, P. Svasek, J. Jansky, F. Paschke, Biotec Seminar Osterreich, Wien, 1987, "Mini-Sensoren in der Bioprozeßtechnik", oral presentation  O. Doblhoff-Dier, 1. Sicherheitsseminar "Bewertung von Einsatzrisiken. Biologische Agenzien", Salzburg 1992, "Grundlagen: Biologische Agenzien", oral presentation  O. Doblhoff-Dier, IACD Users Group, Vienna May 1991, "The Impact of Computer Control on Biotechnology Industry", oral presentation  O. Doblhoff-Dier, Hewlett-Packard Seminar 93, Vienna November 1992, "Moderne Biotechnologie - Prinzipien und Anwendungen", oral presentation  Th. Gaida, O. Doblhoff, H. Katinger, W. Burger, M. Gröschl, E. Benes, "Scale-up of ultrasonic resonance field cell separation devices used in animal cell technology", ESACT Meeting 94, Netherlands  R. Mikler, W. Kramer, O. Doblhoff-Dier, K. Bayer, “Strategies for optimal dissolved oxygen (DO) control“, 6th International Conference on Computer Applications in Biotechnology, Garmisch Partenkirchen, Germany, May 14-17, 1995, poster  O. Doblhoff-Dier, „Fuzzy Control in der Bioverfahrenstechnik“, Informationstagung Mikroelektronik 1995, Vienna, Austria, oral presentation  O. Doblhoff-Dier, „Abscheidung dispergierter Teilchen durch ultraschallinduzierte Koagulation: Anwendung in der tierischen Zellkultur“, Informationstagung Mikroelektronik 1995, Vienna, Austria, poster  O. Doblhoff-Dier, Th. Gaida, W. Burger, M. Gröschl, E. Benes, H. Katinger, „Ultrasonic resonance field cell retention devices: Potentials for large scale mammalian cell culture“, Cell Culture Engineering V, San Diego, California, USA, Jan 28 - Feb 2, 1996  O. Doblhoff-Dier, „Biosafety of Biological Agents - New Developements“, Sept. 17 1996, Blood Transfusion Centre of Slovenia, invited lecture  O. Doblhoff-Dier, „Cell lines: Applications and Biosafety“, Course on Biosafety of Transgenic Organisms in Human Health Products, FIOCRUZ, UNIDO, Rio de Janeiro, Brazil, Oct. 23 - Oct. 27, 1996, invited lecture, course lecturer  O. Doblhoff-Dier, „Prions“, Course on Biosafety of Transgenic Organisms in Human Health Products, FIOCRUZ, UNIDO, Rio de Janeiro, Brazil, Oct. 23 - Oct. 27, 1996, invited lecture, course lecturer  O. Doblhoff-Dier, "Transport of biological agents", 4th Meeting of the European Biosafety Association, Brussells, Belgium, May 30, 1997, invited lecture  O. Doblhoff-Dier, Mini Symposium: Release of biological agents into the environment: Applications and safety evaluation at the Workshop: "Transgenic Organisms in the New Millennium: Risks and Benefits" [Co-sponsored EFB(1) and BATS(2)] Jointly organised by ICGEB and UNIDO, 1 - 5 December 1997, ICGEB - Trieste, Italy (click here to view the program) O. Doblhoff-Dier, "Production of Clinical Trial Material from Animal Cells", Institut für pharmazeutische Chemie, Johann Wolfgang Goethe-Universität, Frankfurt am Main, Germany, Jul. 17, 1997, invited lecture  Otto Doblhoff-Dier, "Production Of Clinical Trial Animal Cell Derived Biologics". invited lecture, Biotechnology Seminar, Thursday, Sept. 11 1997, University of Britisch Columbia, Vancouver, Canada O. Doblhoff-Dier, „Gentechnik in Lebensmitteln“, Annual Meeting of the Austrian Society of Medizinisch Technische AssistentInnen  O. Doblhoff-Dier, "Industrial Enzymes - Biosafety issues", Second Workshop of Experts on Biosafety, March 30. - April 3., 1998, Rio de Janeiro, Brasil  O. Doblhoff-Dier, "Novel Vaccines - Case study: Dengue Fever Vaccines", Second Workshop of Experts on Biosafety, March 30. - April 3., 1998, Rio de Janeiro, Brasil  O. Doblhoff-Dier, "Envionmental Release of Microorganisms", Second Workshop of Experts on Biosafety, March 30. - April 3., 1998, Rio de Janeiro, Brasil  O. Doblhoff-Dier ed., Conference Proceedings: " Implications of a strengthened biological and toxin weapons convention for the biotechnology R&D", A Strengthened Biological and Toxin Weapons Convention (BTWC): Potential Implications for Biotechnology, 28. - 29. May 1998, Institute of Applied Microbiology, Vienna, Austria. Also available on-line: http://www.boku.ac.at/iam/efb/btwcabst.doc O. Doblhoff-Dier, " Possible implications for R&D ", A Strengthened Biological and Toxin Weapons Convention (BTWC): Potential Implications for Biotechnology, 28. - 29. May 1998, Institute of Applied Microbiology, Vienna, Austria. Also available on-line: http://www.boku.ac.at/iam/efb/btwcabst.htm#_Toc422825911 O. Doblhoff-Dier, " Maintaining awareness of developments in biosafety regulations and controls ", A Strengthened Biological and Toxin Weapons Convention (BTWC): Potential Implications for Biotechnology, 28. - 29. May 1998, Institute of Applied Microbiology, Vienna, Austria. Also available on-line: http://www.boku.ac.at/iam/efb/btwcabst.htm#_Toc422825918 O. Doblhoff-Dier, "Gentechnik in Lebensmitteln", Bodensee-Symposium '98, 5.-7. June, Lindau, Germany O. Doblhoff-Dier, "Biological weapons convention - implications for research and industry", European Biosafety Association, 2nd Annual Conference, 20. November 1998, Harlow, UK http://biosafety.ihe.be/EBSA/AC2/Doblhoff.html O. Doblhoff-Dier, "Education and training of biosafety officers, researchers and managers", Biosafety Online Resources,  EU Advanced Workshop on Biosafety, 22.-25.March 1999, TNO, Zeist, Netherlands O. Doblhoff-Dier, "Transgenic animals - concepts and biosafety issues", Online Resources,  EU Advanced Workshop on Biosafety, 22.-25.March 1999, TNO, Zeist, Netherlands O.Doblhoff-Dier, "A strenghtened biological and toxins weapons convention - possible implications for biotechnology", Latin American Biosafety Conference, Rio de Janeiro, Brasil, September 99,  (I Congresso Brasileiro de Biossegurança e I Simpósio Latino Americano de  Produtos Transgênicos de 26 a 29 de setembro de 1999) (download power point slides) (click here to view the program) (click here to view on-line proceedings) O.Doblhoff-Dier, "Risk classification of microorganisms used in environmental biotechnology", Latin American Biosafety Conference, Rio de Janeiro, Brasil, September 99, (I Congresso Brasileiro de Biossegurança e I Simpósio Latino Americano de  Produtos Transgênicos de 26 a 29 de setembro de 1999) (download power point slides) (click here to view the program) (click here to view the on-line proceedings) Doblhoff-Dier, O. "Biosafety: a Critical Review", BioTechnology 2000 - Practice in Non-Food Products, 22-25 October 2000 - Wilhelms-University, Münster, Germany, (click here to view the program) Doblhoff-Dier, O. (2000). How Safe is Biosafe, NFI Seminar. Vienna, Novartis Forschungsinstitut (download power point slides) Doblhoff-Dier, O. (2001). Sicherer Umgang mit biologischen Arbeitsstoffen im Laboratorium, AUVA Fachtagung, Januar 2001, Wien (download power point slides) Doblhoff-Dier, O. (2001). Sicherer Umgang mit biologischen Arbeitsstoffen im Laboratorium, Interlab Tagung, Oktober 2001 Doblhoff-Dier, O. (2001) Die Rolle der Universitäten als Technology Incubators, Expertenmeeting Vienna Valley – Pro Industriestandort Wien, Oktober 2001 (see IV-News)
Organisation of international meetings and courses:	International COMETT Course on "Biological Safety in Industrial and Agricultural Biotechnology", 24. June - 28. June 1996, course director and local organizer  EFB WP Safety in Biotechnology Mini course on "Safety in Biotechnology" at the European Federation Biotechnology Conference ECB 8, Budapest, 1997, course coordinator EFB WP Safety in Biotechnology Mini-symposium "Relsease of biological agents into the environment: Applications and safety evaluation" organised within the UNIDO/ICGEB course on "Transgenic Organisms in the New Millenium: Risks and Benefits" 1 - 5 December 1997, ICGEB - Trieste, Italy , mini symposium coordinator International information and discussion forum on the potential implications for biotechnology R&D and production of the legally binding protocol being negotiated to strengthen the BTWC: "A Strengthened Biological and Toxin Weapons Convention (BTWC): Potential Implications for Biotechnology", 28. - 29. May 1998, Institute of Applied Microbiology, Vienna, Austria, conference chair and local organizer.  Conference proceedings and program available on-line: http://www.boku.ac.at/iam/efb/btwc.htm. The conference was featured in Science, July 3, 1998, 281 p29, Arms control enters the biology lab.(laboratories may be required to submit to inspections under the Biological and Toxin Weapons Convention) Author: Helen Gavaghan. (click here to see a transcript of the article) EU Advanced Workshop on Biosafety under participation of the EFB WP Safety in Biotechnology, 22.-25.March 1999, TNO, Zeist, Netherlands, course coordinator Biotechnology 2000, The World Congress on Biotechnology, 11th International Biotechnology Symposium and Exhibition, 3 - 8 September, 2000, Berlin, Germany, scientific committee, Section VIII: Biotechnology and Society (click here to view program) 10th European Congress on Biotechnology (ECB 10), July 8-11, 2001, Madrid, Spain, scientfic committee (click here to view the program) The European Biosafety Association (EBSA) Annual Conference, Vienna on November 22 and 23, 2001 - postponed to March 2002, local organizer The 5th Gene Delivery & Cellular Protein Expression Conference, Semmering , Austria, 9th and 13th of September, 2001, scientific committee, local organizing team
Slides for Lectures :	Here are some of my private lecture slides in Microsoft Powerpoint format compressed by the ZIP utility. You can use the material for lectures, just please let me know what you think of the quality. Do not use the material for publications as much of it was compiled from other sources and may be copyrighted.  newdev.zip a lecture on new developements in biosafety including animal cell cultures (operator and environmental safety, product safety), prions, emerging infetctious diseases originally presented at the Medical Faculty in Lublijana 96  prions.zip a lecture on prions including aetiology, animal and human prion diseases, inactivation of prions etc. These slides are a subset of the newdev.zip file  cellcult.zip a lecture on biosafety of animal cell cultures (operator and environmental safety) and cell culture derived products (including virus removal validation) originally presented at the biosafety workshop in Rio de Janeiro 96 (brasil99.zip) Lectures on the environmental applicion of microorganisms and on possible implications of a strengthtened biological and toxins weapons convention on biotechnology presented at the Congresso Brasileiro de Biossegurança e I Simpósio Latino Americano de  Produtos Transgênicos (vorlesung.zip) Slides for the course on "Bioprocess Control"  (nfi.ppt) How Safe is Biosafe, NFI Seminar. Vienna, Novartis Forschungsinstitut, 2000 (auva.ppt) Doblhoff-Dier, O. (2001). Sicherer Umgang mit biologischen Arbeitsstoffen im Laboratorium, AUVA Fachtagung, January 2001, Wien (anlagenbau.zip) Slides for the course on "Biotechnology Facilities, Systems and Equipment" (Zmuthgasse.ppt) Presentation of the Biotechnology / Life Science Center of the University for Agriculture (iam.ppt) Presentation slides for the Institute for Applied Microbiology (biosafety.pdf) Manuscript for the biosafety lecture Part 1: Pathogenic species and biosafety - Biologische Agenzien und biologische Sicherheit (in german only) (containment.pdf) Manuscript for the biosafety lecture Part 2: Pathogenic species and biosafety - Biologische Agenzien und biologische Sicherheit (in german only) (Biosafety_deutsch.zip) Powerpoint slides for the biosafety lecture Pathogenic species and biosafety - Biologische Agenzien und biologische Sicherheit (in german only)