Report
30th Month Project and Steering Committee Meeting FUCOMYR - Project
QLKT-2001-02044
Project:
Novel tools for developing Fusarium resistant and toxin free wheat for Europe.
Date
of meeting:
February 23 (9.00-18.00 hours) to February 24, 2004 (9.00 to 14.00 hours)
Place of the meeting: IFA-Tulln,
Tulln, Austria
Time
schedule:
Start 1. day, final 2. day
Prof. P. Ruckenbauer
(PM) (Project coordinator), Dr. U. Scholz (US) (Project manager), Dr. M. Lemmens (ML), Dr. H. Buerstmayr (HB) (WP2 coordinator) (Austria), Prof. A. Mesterhazy (AM)
(WP1 coordinator) (Hungary), Dr. F. Doohan (FD) (WP3 coordinator), Dr. P. Nicholson (PN) (United
Kingdom), Dr. R. Hardwick invited,
present during work package reporting sessions were project partner Dr.Franziska
Loeschenberger (FL)
(Austria) and Dr.
G. Adam (GA).
Program:
1.
Introduction
Welcome by PR;
Appointing of PR and US as chairperson and secretary, respectively.
2.
Approve of last meeting minutes
Last
meeting minutes were unanimously approved.
3.
Matters arising from last meeting
The
following matters arose from last meeting:
a)
Feedback
concerning the successful financial transfer was missing,
b)
we request from the financial officer to brake down the retained amount (to
recover the initial advance) partner wise.
c)
WP1: No limitations for the deliverables and milestones due in year 3 are
foreseen, delays from year 2 will be corrected in year 3. No limitations for the
deliverables and milestones due in year 3 are foreseen.
d)
WP2: Maps
will be refined in the coming season by adding additional SSR markers.
High-resolution mapping populations are now advanced to F6-generation.
Genotyping is underway, phenotyping will start in 2004 as planned.
e)
WP3: Some changes in the ambitious subtask 2 and 3 are suggested in this work
package due to recent developments within and outside the group of researchers.
The Deliverables and milestones due to date are generally achieved, but have
implications for, and suggest that modifications be made to, future work and
milestones.
f) Finances
not yet received (due date March 31, 2004, R. Hardwick
will inquire about it) g) Deadline for reporting
remains tight (therefore 1 month postponement from the of the scientific officer
approved.
h) All should submit their presentation given at the meeting to allow follow up by each partner and co-worker in password protected area of our FUCOMYR website
4. Correspondence and reporting
a) Feedback concerning the successful financial transfer was missing,
b) Respond to request of information in time, c) Follow suggested schedule for reporting
c)
Add all new
colleagues on your mailing list
d)
reporting deadline May 15, 2004, on the website latest May 31, 2004-03-04
e)
Third annual report about third year only (details in management presentation)
5.
Finances
a) please let us know if you received the money for the previous rate
b) break down according to a formula considering overall payment minus 15% and yearly requirements (assistance by financial officer requested)
6.
Detailed discussion of all work packages
WP1
After
a detailed presentation of screening data from 2002 and 2003 the status of
progress in WP1 has been reported by AM and ML (ST1, ST2, ST3, ST4).AM suggests to obtain
additional FDK data for other populations as well. Based on one season data AM
determined strong influence of plant height and anthesis on FHB severity. PR
suggests additional analysis (i.e. rank correlations) and HB to include the 2004
data in the analysis. PN suggests a better phenotyping and resistance definition
to distinguish between pleiotropie and linkage. FHB screening data from
nurseries of P2, P3 and P4 are similar, while results between Szeged and Tulln
differ more. Therefore for a subset of genotypes the inoculation methods of P1
and P5 will be compared in Tulln and Szeged in 2004. ML presents the efficiency
of the 5A QTL against a number of Fusarium species. He observed that F.
sporotrichioides does not cause high FDK values in difference to other
Fusarium species tested. No
limitations for the deliverables and milestones due in year 3 are foreseen. The
presentation of the WP1 results will be accessible in the password protected
area of the FUCOMYR website.
WP2
An overview about the progress within the
work package was given.
Frontana/Remus QTL mapping: At IFA phenotyping and genotyping is
completed. At Szeged (H) one more season of resistance testing is planned. Arina/Riband
QTL mapping: Genotyping was refined with additional SSR markers by SURL.
Phenotyping will be repeated on three sites (UK, A. H) in 2004. High-resolution
mapping: Genotyping of populations
is underway at IFA. Plants are now ripening in the greenhouse of IFA, harvesting
will start soon. Phenotyping on selected recombinants will start in 2004 as
planned. Concerning the 5A QTL we are not sure yet if we are dealing with
several linked genes or a single gene. Maps will be refined in the coming season
by adding additional published SSR markers, which became available recently. We
have to answer how to deal with the flanking marker and to determine, if the QTL
is situated in a low combination area of the chromosome or elsewhere. Genotyping
of screening nursery: Additional SSR markers have been analyzed on 96 lines
of the screening nursery by SURL. Data are available at SURL for genetic
similarity analysis, haplotype analysis and marker trait association analysis.For breeding applications there is the
potential to combine the resistance coming from Frontana with resistance from
Asian sources.We anticipate that the scientific activities
as well as deliverables and milestones of WP 2 within the third project year can
be achieved. The presentation of the WP2 results will be accessible in the
password protected area of the FUCOMYR website.
WP3
FD, GA and PN reported about progress in WP3. The approved adjustments can be viewed in the password protected area of the FUCOMYR website. ST1: In the detached leaf assay correlations between latent period and FHB in commercial lines are functional. The SE microscopy work is underway. The Mycotoxin part of the detached leaf assays remains to be done until the end of the third project year. ST2: Full-length clones were obtained from a cv. Frontana cDNA library and we are currently trying to sequence these clones. We have sequenced two genes and are currently sequencing three more such genes. An analysis of the sequence data will be presented in the sixth report. Expression of some transcripts is higher in F. culmorum-inoculated cv. CM 82036 than in cv. Remus heads. PN reported, that the DH population was tested over two years for AUDPC, RSW, DON, FDNA. The 6B QTL was significant for several traits in both years. Populations were sown for new test in poly-tunnel and population was sent to Austria and Hungary for field testing. Concerning the ribosomal protein L3, allele-specific primers for 5A and 5D are used, and for Group 4 new primers developed. Some specific PDR sequences are generally increased in response to DON in FHB resistant and susceptible varieties. The accumulation of DON was reduced in grain extracts of some accessions of the mapping populations CM82036/Remus.K. Ansari, the Ph.D. student of FD will work for few month in GA laboratory in Austria continuing his work with candidate resistance genes. GA reported that work has begun on the wheat cDNA library for yeast expression studies.
The
Deliverables and milestones are achieved, and the approved adjustments will be
met. The presentations of the WP3 results will be accessible in the password
protected area of the FUCOMYR
website.
Joint
dinner
7. Technological implementation plan
The
information based on the web based version of eTIP due at the end of the project
was presented. Besides tasks under sole responsibility of the project
coordinator, several points have to be addressed in a joint approach. Further,
each partner has to fill out its own questionnaire towards the end of the
project. Please
view for details the management presentation.
The part concerning the right to Veto
decisions of the consortium will be removed from the agreement. If all partner
completed the part of the pre-existing knowledge, the CA will be distributed to
each partner ready to be signed.
9.
Other motions
Views, ideas,
concerns and opportunities regarding the participation of our consortium within
the 6th EU-Framework program were exchanged.
10. Any other business
The
project website was accessed – now located at the BOKU server - within
the last three months 1385 times. Comments for any improvements were invited. A
project flyer on glossy paper was not considered as necessary.
11.
Next meeting
The
next meeting is the 36th month meeting in Hungary, where the
participation of all project partner is required. September 27th and
28th, 2004 has been set as meeting date. It has been suggested to
participate in the joint meeting of the US Wheat and Barley Scab initiative
together with the European Fusarium Seminar in December 2004. The opinion to
move the 36th month meeting in Norwich from February 2005 to Orlando,
Florida by 2 month and use partly resource available for the steering committee
meeting was discussed. The position of each partner will be requested to
safeguard fairness within the consortium.
12. Laboratory tour
The
laboratory tour covered greenhouses and laboratories, where phytopathological
and molecular methods relevant for the project were presented.
End
of meeting