970050 Development of immunoanalytical testsystems for food analysis


Type
Exercise course
Semester hours
2
Lecturer (assistant)
Organisation
Department of Agrobiotechnology, IFA-Tulln
Offered in
Wintersemester 2016/17
Languages of instruction
Englisch

Content

Development of Enzyme Immunosorbent Assays for the usage in Food Analysis: Immunisation strategies, development of polyclonal and monoclonal antibodies, preparation of microtiterplates, ELISA-formats, validation guidelines for ELISAs for food analysis.

Previous knowledge expected

Principles in biochemistry according to structure and composition of proteins, especially enzymes and antibodies.

Objective (expected results of study and acquired competences)

Draft knowledge about different possibilities for antibody develpment. To establish understanding about ELISA-development. Distinguish between the different possible ELISA-formats available. Differentiation, illustration and discussion about the obtained results from the conducted experiments.
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