752339 Protein engineering


Type
Lecture and exercise
Semester hours
2
Lecturer (assistant)
Organisation
Institute of Food Technology
Offered in
Wintersemester 2016/17
Languages of instruction
Englisch

Content

Mutagenesis is performed with a plasmid containing the gene for pyranose 2-oxidase. After transformation in a suitable expression host (E. coli), colonies are transferred to 96 well plates, induced, grown overnight, and the cells lysed to release the mutated enzyme. This crude extracts are screened for improved thermo stability and altered substrate specificity. Mutants with improved properties are selected and sequenced.
.
ATTENTION: the times in the seminar room reflect only the reservation for this room, the course is essentially a full-day-course from 9 am until 5 pm.

Previous knowledge expected

Knowledge of microbiology/molecular biology highly is required
.

Objective (expected results of study and acquired competences)

There is an increasing demand for improved enzymes for industrial application, e.g. improved thermo stability, and altered substrate specificity. The methods of choice for producing this enzymes are directed evolution, site directed mutagenesis, gene shuffling, and a mixture of this methods. Aim of the course is an understanding of the principles of these methods, knowledge about what methods are recommended under what circumstances, and practical competence in applying one method in the lab.
.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.