791416 Instructional course IVB - next-generation sequencing

Exercise course
Semester hours
Lecturer (assistant)
Stralis-Pavese, Nancy , Himmelbauer, Heinz , Dohm, Juliane
Institute of Biotechnology
Offered in
Wintersemester 2017/18
Languages of instruction


This course will introduce the students to next-generation sequencing (NGS) technologies, combining theoretical sessions illustrating sequencing concepts, principal library preparation methods, and data analysis strategies. Practical work will start with quality control (QC) and quantitation of genomic DNA, followed by preparation and QC of a genomic library for sequencing on the Illumina NGS platform. Bioinformatics work will include an introduction to read filtering, read mapping, and the analysis of genomic and transcriptome sequencing data.

Objective (expected results of study and acquired competences)

The course will provide students with an introduction and an overview to the rapidly developing field of NGS. The students will be trained to realize the perspectives how NGS data can impact their research. Based on the knowledge they acquire, the students will be able to make decisions which sequencing strategy to choose for particular research questions, and how to avoid and solve problems frequently encountered during NGS data analysis.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.