791369 Practical course in cell culture and fermentation


Type
Exercise course
Semester hours
3
Lecturer (assistant)
Mayrhofer, Patrick , Schosserer, Markus , Borth, Nicole , Kunert, Renate , Kasper, Cornelia , Burk-Luibl, Janina , Egger, Dominik
Organisation
Institute of Biotechnology
Offered in
Sommersemester 2018
Languages of instruction
Englisch

Content

!!!! Check expected previous knowledge before registering !!!!



Part "3 D cell culture"
Seeding of 3 D matrix with model cell line
determination of:
Viability (MTT)
Growth curve
Cell nuclei staining (DAPI)

Partl "Transfection, Characterization and Imaging of Mammalian Cells":
Transfection of Hela cells with plasmids coding for fluorescent proteins
Immunofluorescence staining for cell-type specific markers
Chromosome preparation
Microscopy
Discussion and interpretation of results

Part "Production of Proteins"
Transfection of mammalian cell lines
Isolation of a recombinant cell line
Batch cultivation
cellular analysis (Microscopy, ELISA, FACS)
Data Evaluation and Interpretation

Previous knowledge expected

accepted are only master and PhD students
Bacchelorstudents are not accepted
Additionally one of the following examinations must be passed with a positive mark:
Zellfabriken (791351) or Animal Cell Culture (791318) or Stem cells and tissue engineering (791367)

Objective (expected results of study and acquired competences)

After completion of this course students understand cell culture technology and its possible applications.
They are able to handle mammalian cell cultures using both primary and established cell lines
Students can design cell culture experiments and interpret the analyzed data.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.