791356 Metabolic and cell engineering


Type
Lecture
Semester hours
2
Lecturer (assistant)
Mattanovich, Diethard
Organisation
Institute of Biotechnology
Offered in
Sommersemester 2018
Languages of instruction
Englisch

Content

Summary and update of cellular metabolism: catabolism and anabolism, energy conversion, reduction and oxidation equvalents.
Balances and equations describing cellular processes: mass, energy, redox and carbon balances; basic models to describe cells.
Application of metabolic modelling for metabolic and cell engineering.
Systems biology: describing the complexity of cellular systems.
Methods: metabolic flux analysis, metabolomics, transcriptomics, quantitative proteomics.
Tools for metabolic and cell engineering.
Major biotechnological challanges: New metabolic pathways leading to new products. Microbial stress during production. Protein secretion.
Applications of metabolic engineering: overproduction of metabolites with microorganisms.
Applications of cell engineering: improving cellular properties, e.g. secretion of recombinant proteins, strain robustness (stress resistance).

Previous knowledge expected

Biochemistry and cellular physiology. Metabolic pathways and metabolic regulation. Metabolic modelling.

Objective (expected results of study and acquired competences)

Students are familiar with principles of designing metabolic and cell engineering projects.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.