Viršček Marn

Die Bodenkultur - Journal for Land Management, Food and Environment

M. Viršček Marn and Mavric:

Factors affecting the reliability of PDV and PNRSV detection in peach by DAS-ELISA


To ensure reliable serological testing of stone fruits in Slovene growing conditions, several factors influencing the detection of PDV and PNRSV by DAS-ELISA were studied. Various tissues were sampled at different dates in 2003 and 2004 on PDV and PNRSV infected peach trees of cv. Veteran and analysed by DAS-ELISA. Mean absorbance values as well as the variability of results within analysis differed depending on the date of analysis, analysed tissue and virus.

Both viruses could be reliably detected by DAS-ELISA in winter and autumn buds and in flowers or young leaves in spring. A deteriorating effect of high temperatures on concentrations of PNRSV and PDV in leaves has been observed.

Key words: PDV, PNRSV, DAS-ELISA, high temperature, peach.