Die Bodenkultur - Journal for Land Management, Food and Environment

S. Kereša, M. Barić, H. Šarčević and S. Marchetti:

Callus induction and plant regeneration from immature and mature embryos and immature inflorescences of eight Croatian winter wheat cultivars (Triticum aestivum L.)


Eight Croatian winter wheat cultivars (Triticum aestivum L.) were evaluated for their ability to produce callus and to regenerate whole plants from immature and mature embryos and immature inflorescences. The highest mean percentage regeneration across cultivars (21.6 %) was obtained when immature embryos were used as explant with the highest regeneration of cultivar Zˇitarka (57 %) and Edita (54 %). However, some cultivars regenerated equally efficiently (Edita) or better (Lipa) when immature inflorescences were used. The highest regeneration capacity from mature embryos (26 % for cultivar Magdalen) was much below the best regeneration capacities from the immature embryo or inflorescence culture. As far as the media provided with 2,4-D were used for callus induction from immature embryos, plant regeneration was significantly better than in a medium with picloram. With immature inflorescences, on the contrary, the best culture response was obtained using medium containing picloram.

Key words: winter wheat, embryo culture, immature inflorescences, plant regeneration.